This is helpful for restricting the amplification only to mRNA. It's also possible to exclude these primers if you need to amplify mRNA plus the corresponding genomic DNA. Exon junction match

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Swimming pools of pre-built primers may help generate comprehensive expression profiles, especially where assay sensitivity is critically essential.

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Frequent sequences of Brief DNA oligonucleotides are sometimes employed as primers for sequencing cloned genes (Sanger sequencing). Pick from our usually utilized sequencing primers to initiate your sequence verification tasks.

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Aid This specifies the max amplicon dimensions for the PCR focus on being detected by Primer-BLAST. Usually, the non-unique targets come to be less of a priority if their dimensions are quite substantial considering the fact that PCR is a lot less productive for much larger amplicons. Make it possible for splice variants